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验证数据展示

  • WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

    WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83009-4-PBS

    IF Staining of HepG2 using 83009-4-PBS

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-PBS, Clone: 230523A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83009-4-RR (same clone as 83009-4-PBS)

    IF Staining of HeLa using 83009-4-RR (same clone as 83009-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)

    IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83009-4-RR

    Affinity and Kinetic Characterization of 83009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

golgin 97 Recombinant monoclonal antibody, PBS Only

golgin 97 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83009-4-PBS

产品说明书

CloneNo. 230523A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

GOLGA1, 230523A5, Golgin subfamily A member 1, Golgin-97

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83009-4-PBS targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3335

Product name: Recombinant human GOLGA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-767 aa of BC032853

Sequence: QIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名 GOLGA1, 230523A5, Golgin subfamily A member 1, Golgin-97
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ92805
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.

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