验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, LNCaP cells, MCF-7 cells, SMMC-7721 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83009-4-RR targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | golgin 97 fusion protein Ag3335 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | golgi autoantigen, golgin subfamily a, 1 |
Synonyms | GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5 |
Calculated Molecular Weight | 767 aa, 88 kDa |
Observed Molecular Weight | 97 kDa |
GenBank Accession Number | BC032853 |
Gene Symbol | golgin 97 |
Gene ID (NCBI) | 2800 |
RRID | AB_3670753 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q92805 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.
实验方案
Product Specific Protocols | |
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WB protocol for golgin 97 antibody 83009-4-RR | Download protocol |
IHC protocol for golgin 97 antibody 83009-4-RR | Download protocol |
IF protocol for golgin 97 antibody 83009-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |