golgin 97 Recombinant antibody

golgin 97 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83009-4-RR
CloneNo. 230523A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, LNCaP cells, MCF-7 cells, SMMC-7721 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83009-4-RR targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen golgin 97 fusion protein Ag3335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
RRIDAB_3670753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
WB protocol for golgin 97 antibody 83009-4-RRDownload protocol
IHC protocol for golgin 97 antibody 83009-4-RRDownload protocol
IF protocol for golgin 97 antibody 83009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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