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验证数据展示

  • WB analysis of NK-92 using 87707-3-RR

    WB analysis of NK-92 using 87707-3-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 87707-3-RR (Granulysin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Recombinant protein using 87707-3-RR

    WB analysis of Recombinant protein using 87707-3-RR

    Granulysin Recombinant protein Eg4482 was subjected to SDS PAGE followed by western blot with 87707-3-RR (Granulysin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsil using 87707-3-RR

    IHC staining of human tonsil using 87707-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 87707-3-RR (GNLY/Granulysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsil using 87707-3-RR

    IHC staining of human tonsil using 87707-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 87707-3-RR (GNLY/Granulysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 87707-3-RR

    Affinity of 87707-3-RR

    Biolayer interferometry (BLI) kinetic assays of 87707-3-RR against Human GNLY/Granulysin were performed. The affinity constant is 1.03 nM.

GNLY/Granulysin Recombinant monoclonal antibody

GNLY/Granulysin Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87707-3-RR

产品说明书

CloneNo. 252602F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GNLY, Granulysin, LAG2, Lymphokine LAG-2, NKG5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNK-92 cells, Recombinant protein
Positive IHC detected inhuman tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87707-3-RR targets GNLY/Granulysin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3381

Product name: Recombinant human Granulysin protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-145 aa of NM_006433.5

Sequence: RLSPEYYDLARAHLRDEEKSCPCLAQEGPQGDLLTKTQELGRDYRTCLTIVQKLKKMVDKPTQRSVSNAATRVCRTGRSRWRDVCRNFMRRYQSRVTQGLVAGETAQQICEDLRLCIPSTGPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 granulysin
别名 GNLY, Granulysin, LAG2, Lymphokine LAG-2, NKG5
计算分子量16 kDa
观测分子量14-15 kDa
GenBank蛋白编号NM_006433.5
基因名称 GNLY
Gene ID (NCBI) 10578
RRIDAB_3745708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22749-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granulysin is a cationic, saposin-like antimicrobial protein expressed in cytotoxic granules of human CD8+ T cells and NK cells. It exists as 9-kDa (cytolytic) and 15-kDa (secreted) isoforms, killing intracellular pathogens and tumor cells by disrupting membranes or inducing ER stress-mediated apoptosis.(PMID: 17596262;17182542)

实验方案

Product Specific Protocols
IHC protocol for GNLY/Granulysin antibody 87707-3-RRDownload protocol
WB protocol for GNLY/Granulysin antibody 87707-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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