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验证数据展示

  • WB analysis of NK-92 using 87707-3-RR (same clone as 87707-3-PBS)

    WB analysis of NK-92 using 87707-3-RR (same clone as 87707-3-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 87707-3-RR (Granulysin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87707-3-PBS in a different storage buffer formulation.

  • WB analysis of Recombinant protein using 87707-3-RR (same clone as 87707-3-PBS)

    WB analysis of Recombinant protein using 87707-3-RR (same clone as 87707-3-PBS)

    Granulysin Recombinant protein Eg4482 was subjected to SDS PAGE followed by western blot with 87707-3-RR (Granulysin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87707-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsil using 87707-3-RR (same clone as 87707-3-PBS)

    IHC staining of human tonsil using 87707-3-RR (same clone as 87707-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 87707-3-RR (GNLY/Granulysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87707-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsil using 87707-3-RR (same clone as 87707-3-PBS)

    IHC staining of human tonsil using 87707-3-RR (same clone as 87707-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 87707-3-RR (GNLY/Granulysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87707-3-PBS in a different storage buffer formulation.

  • Affinity of 87707-3-RR

    Affinity of 87707-3-RR

    Biolayer interferometry (BLI) kinetic assays of 87707-3-RR against Human GNLY/Granulysin were performed. The affinity constant is 1.03 nM.

GNLY/Granulysin Recombinant monoclonal antibody, PBS Only

GNLY/Granulysin Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87707-3-PBS

产品说明书

CloneNo. 252602F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

GNLY, Granulysin, LAG2, Lymphokine LAG-2, NKG5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87707-3-PBS targets GNLY/Granulysin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3381

Product name: Recombinant human Granulysin protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-145 aa of NM_006433.5

Sequence: RLSPEYYDLARAHLRDEEKSCPCLAQEGPQGDLLTKTQELGRDYRTCLTIVQKLKKMVDKPTQRSVSNAATRVCRTGRSRWRDVCRNFMRRYQSRVTQGLVAGETAQQICEDLRLCIPSTGPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 granulysin
别名 GNLY, Granulysin, LAG2, Lymphokine LAG-2, NKG5
计算分子量16 kDa
观测分子量14-15 kDa
GenBank蛋白编号NM_006433.5
基因名称 GNLY
Gene ID (NCBI) 10578
RRIDAB_3745708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22749-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Granulysin is a cationic, saposin-like antimicrobial protein expressed in cytotoxic granules of human CD8+ T cells and NK cells. It exists as 9-kDa (cytolytic) and 15-kDa (secreted) isoforms, killing intracellular pathogens and tumor cells by disrupting membranes or inducing ER stress-mediated apoptosis.(PMID: 17596262;17182542)

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