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验证数据展示

  • IF Staining of HepG2 using CL488-67497

    IF Staining of HepG2 using CL488-67497

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GNB3 antibody (CL488-67497, Clone: 2B6E1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated GNB3 Monoclonal antibody

GNB3 Monoclonal Antibody for IF/ICC
Cat No. CL488-67497

产品说明书

CloneNo. 2B6E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

IF/ICC

别名

GNB3, Transducin beta chain 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67497-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67497 targets GNB3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原

CatNo: Ag7050

Product name: Recombinant human GNB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-340 aa of BC002454

Sequence: EMEQLRQEAEQLKKQIADARKACADVTLAELVSGLEVVGRVQMRTRRTLRGHLAKIYAMHWATDSKLLVSASQDGKLIVWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNMCSIYNLKSREGNVKVSRELSAHTGYLSCCRFLDDNNIVTSSGDTTCALWDIETGQQKTVFVGHTGDCMSLAVSPDFNLFISGACDASAKLWDVREGTCRQTFTGHESDINAICFFPNGEAICTGSDDASCRLFDLRADQELICFSHESIICSITSVAFSLSGRLLFAGYDDFNCNVWDSMKSERVGILSGHDNRVSCLGVTADGMAVATGSWDSFLKIWN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 GNB3, Transducin beta chain 3
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC002454
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_3084352
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16520
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GNB3 antibody CL488-67497Download protocol
Standard Protocols
Click here to view our Standard Protocols