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验证数据展示

  • WB analysis using 67497-1-Ig

    WB analysis using 67497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67497-1-Ig

    WB analysis of HepG2 using 67497-1-Ig

    WB result of GNB3 antibody (67497-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNB3 transfected HepG2 cells.

  • IHC staining of human liver cancer using 67497-1-Ig

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67497-1-Ig

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67497-1-Ig

    IF Staining of HepG2 using 67497-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • KD/KO验证

GNB3 Monoclonal antibody

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67497-1-Ig

产品说明书

CloneNo. 2B6E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

2B6E1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, HepG2 cells, L02 cells, pig brain tissue, rat retina tissue, Raji cells, Ramos cells, Daudi cells, RKO cells, Karpas-422 cells, IMR-32 cells, Rat brain cells, Mouse brain cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67497-1-Ig targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag7050

Product name: Recombinant human GNB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-340 aa of BC002454

Sequence: EMEQLRQEAEQLKKQIADARKACADVTLAELVSGLEVVGRVQMRTRRTLRGHLAKIYAMHWATDSKLLVSASQDGKLIVWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNMCSIYNLKSREGNVKVSRELSAHTGYLSCCRFLDDNNIVTSSGDTTCALWDIETGQQKTVFVGHTGDCMSLAVSPDFNLFISGACDASAKLWDVREGTCRQTFTGHESDINAICFFPNGEAICTGSDDASCRLFDLRADQELICFSHESIICSITSVAFSLSGRLLFAGYDDFNCNVWDSMKSERVGILSGHDNRVSCLGVTADGMAVATGSWDSFLKIWN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 2B6E1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC002454
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_2882721
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
IF protocol for GNB3 antibody 67497-1-IgDownload protocol
IHC protocol for GNB3 antibody 67497-1-IgDownload protocol
WB protocol for GNB3 antibody 67497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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