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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 87127-3-RR (same clone as 87127-3-PBS)

    WB analysis using 87127-3-RR (same clone as 87127-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87127-3-RR (GNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87127-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87127-3-RR (same clone as 87127-3-PBS)

    IF Staining of HepG2 using 87127-3-RR (same clone as 87127-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (87127-3-RR, Clone: 252318D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87127-3-PBS in a different storage buffer formulation.

  • Affinity of 87127-3-RR

    Affinity of 87127-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87127-3-RR against Human GNB1 were performed. The affinity constant is 0.353 nM.

GNB1 Recombinant monoclonal antibody, PBS Only

GNB1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87127-3-PBS

产品说明书

CloneNo. 252318D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

GBB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® GNB1 Ready-To-Use IHC Kit. GNB1 primary antibody included.

产品信息

87127-3-PBS targets GNB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0313

Product name: Recombinant human GNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-339 aa of BC004186

Sequence: SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 1
别名 GBB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
计算分子量 37 kDa
观测分子量35 kDa
GenBank蛋白编号BC004186
基因名称 GNB1
Gene ID (NCBI) 2782
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62873
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

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