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验证数据展示

  • WB analysis using 87127-3-RR

    WB analysis using 87127-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87127-3-RR (GNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 87127-3-RR

    IF Staining of HepG2 using 87127-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (87127-3-RR, Clone: 252318D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87127-3-RR

    Affinity of 87127-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87127-3-RR against Human GNB1 were performed. The affinity constant is 0.353 nM.

GNB1 Recombinant monoclonal antibody

GNB1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87127-3-RR

产品说明书

CloneNo. 252318D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

GBB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, T-47D cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® GNB1 Ready-To-Use IHC Kit. GNB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87127-3-RR targets GNB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0313

Product name: Recombinant human GNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-339 aa of BC004186

Sequence: SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 1
别名 GBB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
计算分子量 37 kDa
观测分子量35 kDa
GenBank蛋白编号BC004186
基因名称 GNB1
Gene ID (NCBI) 2782
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

实验方案

Product Specific Protocols
IF protocol for GNB1 antibody 87127-3-RRDownload protocol
WB protocol for GNB1 antibody 87127-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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