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验证数据展示

  • WB analysis of rat kidney using 10864-2-AP (same clone as 10864-2-PBS)

    WB analysis of rat kidney using 10864-2-AP (same clone as 10864-2-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GM2A antibody included
  • IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GM2A antibody included
  • IP experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    IP experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    IP result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    FC experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GM2A Polyclonal antibody, PBS Only

GM2A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 10864-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® GM2A Ready-To-Use IHC Kit. GM2A primary antibody included.

产品信息

10864-2-PBS targets GM2A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1295

Product name: Recombinant human GM2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC009273

Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GM2 ganglioside activator
别名
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009273
基因名称 GM2A
Gene ID (NCBI) 2760
RRIDAB_2110807
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17900
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

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