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验证数据展示

  • WB analysis using 18884-1-AP

    WB analysis using 18884-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18884-1-AP (Glutaredoxin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 18884-1-AP

    IF Staining of MCF-7 using 18884-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Glutaredoxin 5 antibody (18884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Glutaredoxin 5 Polyclonal antibody

Glutaredoxin 5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18884-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

GRX5, Glutaredoxin-related protein 5, mitochondrial, GLRX5, GLRX 5, FLB4739

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, mouse heart tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18884-1-AP targets Glutaredoxin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13445

Product name: Recombinant human GLRX5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-157 aa of BC023528

Sequence: MSGSLGRAAAALLRWGRGAGGGGLWGPGVRAAGSGAGGGGSAEQLDALVKKDKVVVFLKGTPEQPQCGFSNAVVQILRLHGVRDYAAYNVLDDPELRQGIKDYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIHSTLLDEKKDQDSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaredoxin 5
别名 GRX5, Glutaredoxin-related protein 5, mitochondrial, GLRX5, GLRX 5, FLB4739
计算分子量 157 aa, 17 kDa
观测分子量10-15 kDa
GenBank蛋白编号BC023528
基因名称 GLRX5
Gene ID (NCBI) 51218
RRIDAB_3669306
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86SX6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation.

实验方案

Product Specific Protocols
IF protocol for Glutaredoxin 5 antibody 18884-1-APDownload protocol
WB protocol for Glutaredoxin 5 antibody 18884-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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