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GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B7A8

Cat No : 68242-1-Ig

Print datasheet

Synonyms

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

验证数据展示

  • WB analysis using 68242-1-Ig

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68242-1-Ig

    WB analysis of LNCaP using 68242-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68242-1-Ig

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy GLO1 Ready-To-Use IHC Kit. GLO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68242-1-Ig targets GLO1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Rat, Mouse
Immunogen GLO1 fusion protein Ag7314 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glyoxalase I
Synonyms Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-25 kDa
GenBank Accession NumberBC001741
Gene Symbol GLO1
Gene ID (NCBI) 2739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.

实验方案

Product Specific Protocols
WB protocol for GLO1 antibody 68242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols