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验证数据展示

  • FC experiment of HeLa using CL488-67858

    FC experiment of HeLa using CL488-67858

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GIT2 (CL488-67858, Clone:2D9C5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67858

产品说明书

CloneNo. 2D9C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

FC (Intra)

别名

GIT2, GRK-interacting protein 2, KIAA0148, 2D9C5, ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT 2, CAT2, CAT-2, Cool-interacting tyrosine-phosphorylated protein 2, G protein-coupled receptor kinase-interactor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GIT2 Ready-To-Use IHC Kit. GIT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67858-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67858 targets GIT2 in FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 GIT2 fusion protein Ag0321 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 G protein-coupled receptor kinase interacting ArfGAP 2
别名 GIT2, GRK-interacting protein 2, KIAA0148, 2D9C5, ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT 2, CAT2, CAT-2, Cool-interacting tyrosine-phosphorylated protein 2, G protein-coupled receptor kinase-interactor 2
计算分子量 95 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC001379
基因名称 GIT2
Gene ID (NCBI) 9815
RRIDAB_2934576
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14161
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GIT2 antibody CL488-67858Download protocol
Standard Protocols
Click here to view our Standard Protocols