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GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D9C5

Cat No : 67858-1-Ig

Print datasheet

Synonyms

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

验证数据展示

  • WB analysis using 67858-1-Ig

    WB analysis using 67858-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human prostate cancer using 67858-1-Ig

    IHC staining of human prostate cancer using 67858-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat
Immunogen GIT2 fusion protein Ag0321 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name G protein-coupled receptor kinase interacting ArfGAP 2
Synonyms ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC001379
Gene Symbol GIT2
Gene ID (NCBI) 9815
RRIDAB_2918616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GIT2 antibody 67858-1-IgDownload protocol
IHC protocol for GIT2 antibody 67858-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols