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验证数据展示

  • WB analysis using 67858-1-Ig

    WB analysis using 67858-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human prostate cancer using 67858-1-Ig

    IHC staining of human prostate cancer using 67858-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GIT2 antibody included

GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67858-1-Ig

产品说明书

CloneNo. 2D9C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, ELISA

别名

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GIT2 Ready-To-Use IHC Kit. GIT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag0321

Product name: Recombinant human GIT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 254-462 aa of BC001379

Sequence: DSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 G protein-coupled receptor kinase interacting ArfGAP 2
别名 ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148
计算分子量 95 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC001379
基因名称 GIT2
Gene ID (NCBI) 9815
RRIDAB_2918616
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14161
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for GIT2 antibody 67858-1-IgDownload protocol
WB protocol for GIT2 antibody 67858-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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