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验证数据展示

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

  • WB analysis using 98262-1-RR

    WB analysis using 98262-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed

  • IHC staining of Transfected HEK-293T using 98262-1-RR

    IHC staining of Transfected HEK-293T using 98262-1-RR

    Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens).

  • IF Staining of Transfected HEK-293T using 98262-1-RR

    IF Staining of Transfected HEK-293T using 98262-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Anti- GGGGS Linker Rabbit Recombinant Antibody

GGGGS Linker Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC
Cat No. 98262-1-RR

产品说明书

CloneNo. 242306C8

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC

别名

G4S, G4S link, G4S Linker, GGGGS, GS Linker

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman SCFV Lv-Hv转染 Transfected HEK-293T cells
Positive IHC detected inTransfected HEK-293T cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTransfected HEK-293T cells
Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:40000-1:160000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC Application Description
经测试反应性 n/a
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GGGGS Linker
别名 G4S, G4S link, G4S Linker, GGGGS, GS Linker
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody was raised against a synthetic peptide (GGGGSGGGGSGGGGS).

实验方案

Product Specific Protocols
WB protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
IHC protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
IF protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols