验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293T cells |
| Positive IHC detected in | Transfected HEK-293T cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Transfected HEK-293T cells, Transfected CHO-K1 |
| Positive FC detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:40000-1:160000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IHC, IF/ICC, FC Application Description |
| 经测试反应性 | recombinant protein |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | GGGGS Linker |
| 别名 | G4S, G4S link, G4S Linker, GGGGS, GS Linker |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IF protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IHC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| WB protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
