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验证数据展示

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

  • WB analysis using 98262-1-RR

    WB analysis using 98262-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed

  • FC experiment of Transfected HEK-293T using 98262-1-RR

    FC experiment of Transfected HEK-293T using 98262-1-RR

    1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed

  • IHC staining of Transfected HEK-293T using 98262-1-RR

    IHC staining of Transfected HEK-293T using 98262-1-RR

    Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens).

  • IF Staining of Transfected HEK-293T using 98262-1-RR

    IF Staining of Transfected HEK-293T using 98262-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 98262-1-RR

    Affinity and Kinetic Characterization of 98262-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98262-1-RR against scFv (single chain fragment variable) format with variable regions of heavy (VH) and light (VL) chains joined together by G4S linker were performed. The affinity constant is 3.87 nM.

Anti- GGGGS Linker Rabbit Recombinant Antibody

GGGGS Linker Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC
Cat No. 98262-1-RR

产品说明书

CloneNo. 242306C8

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC

别名

G4S, G4S link, G4S Linker, GGGGS, GS Linker

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Positive IHC detected inTransfected HEK-293T cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTransfected HEK-293T cells
Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:40000-1:160000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC Application Description
经测试反应性 n/a
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GGGGS Linker
别名 G4S, G4S link, G4S Linker, GGGGS, GS Linker
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody was raised against a synthetic peptide (GGGGSGGGGSGGGGS).

实验方案

Product Specific Protocols
WB protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
IHC protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
IF protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
FC protocol for GGGGS Linker antibody 98262-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols