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验证数据展示

  • FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • WB analysis using 98262-1-RR (same clone as 98262-1-PBS)

    WB analysis using 98262-1-RR (same clone as 98262-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • IHC staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    IHC staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    IF Staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected CHO-K1 using 98262-1-RR (same clone as 98262-1-PBS)

    IF Staining of Transfected CHO-K1 using 98262-1-RR (same clone as 98262-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected CHO-K1 using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98262-1-RR

    Affinity and Kinetic Characterization of 98262-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98262-1-RR against scFv (single chain fragment variable) format with variable regions of heavy (VH) and light (VL) chains joined together by G4S linker were performed. The affinity constant is 3.87 nM.

Anti-GGGGS Linker Rabbit Recombinant Antibody, PBS Only

GGGGS Linker Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC
Cat No. 98262-1-PBS

产品说明书

CloneNo. 242306C8

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, IHC, IF/ICC, FC

别名

G4S, G4S link, G4S Linker, GGGGS, GS Linker

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98262-1-PBS targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IHC, IF/ICC, FC Application Description
经测试反应性 recombinant protein
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GGGGS Linker
别名 G4S, G4S link, G4S Linker, GGGGS, GS Linker
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.

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