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验证数据展示

  • WB analysis of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    WB analysis of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • WB analysis of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    WB analysis of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    IF Staining of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    IF Staining of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86609-3-RR

    Affinity and Kinetic Characterization of 86609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86609-3-RR against Human GFPT2 were performed. The affinity constant is below 1 pM.

GFPT2 Recombinant monoclonal antibody, PBS Only

GFPT2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86609-3-PBS

产品说明书

CloneNo. 251454E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86609-3-PBS targets GFPT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7275

Product name: Recombinant human GFPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-682 aa of BC000012

Sequence: IFEQPESVFNTMRGRVNFETNTVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVTNTVGSSISRETDCGVHINAGPEVGVASTKAYTSQFISLVMFGLMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamine-fructose-6-phosphate transaminase 2
别名 D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2
计算分子量 77 kDa
观测分子量 75-77 kDa
GenBank蛋白编号BC000012
基因名称 GFPT2
Gene ID (NCBI) 9945
RRIDAB_3745004
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94808
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

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