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验证数据展示

  • WB analysis of NIH/3T3 using 86609-3-RR

    WB analysis of NIH/3T3 using 86609-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 86609-3-RR

    WB analysis of A375 using 86609-3-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 86609-3-RR

    IF Staining of A375 using 86609-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of NIH/3T3 using 86609-3-RR

    IF Staining of NIH/3T3 using 86609-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86609-3-RR

    Affinity and Kinetic Characterization of 86609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86609-3-RR against Human GFPT2 were performed. The affinity constant is below 1 pM.

GFPT2 Recombinant monoclonal antibody

GFPT2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86609-3-RR

产品说明书

CloneNo. 251454E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A375 cells
Positive IF/ICC detected inNIH/3T3 cells, A375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86609-3-RR targets GFPT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7275

Product name: Recombinant human GFPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-682 aa of BC000012

Sequence: IFEQPESVFNTMRGRVNFETNTVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVTNTVGSSISRETDCGVHINAGPEVGVASTKAYTSQFISLVMFGLMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamine-fructose-6-phosphate transaminase 2
别名 D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2
计算分子量 77 kDa
观测分子量 75-77 kDa
GenBank蛋白编号BC000012
基因名称 GFPT2
Gene ID (NCBI) 9945
RRIDAB_3745004
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94808
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

实验方案

Product Specific Protocols
IF protocol for GFPT2 antibody 86609-3-RRDownload protocol
WB protocol for GFPT2 antibody 86609-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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