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CoraLite® Plus 488-conjugated GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-50430

Print datasheet

Synonyms

CFP, eGFP, eYFP, GFP, GFP-tag, green, green fluorescence protein, green fluorescent protein, RS-GFP, S65T-GFP, YFP

验证数据展示

  • IF Staining of mouse brain using CL488-50430

    IF Staining of mouse brain using CL488-50430

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-50430

    IF Staining of mouse brain using CL488-50430

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-50430 targets GFP tag in IF applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Applications IF-P
Tested Reactivity aequorea victoria, recombinant protein
Immunogen GFP tag fusion protein Ag2128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GFP tag
Synonyms CFP, eGFP, eYFP, GFP, GFP-tag, green, green fluorescence protein, green fluorescent protein, RS-GFP, S65T-GFP, YFP
Calculated Molecular Weight 26 kDa
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

This antibody is labeled with CoraLite Plus 488.


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GFP tag antibody CL488-50430Download protocol
Standard Protocols
Click here to view our Standard Protocols