|Positive WB detected in||recombinant protein, Transfected HEK-293 cells|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
50430-2-AP targets GFP tag in WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.
|Tested Applications||IF, IP, WB, ELISA|
|Cited Applications||ChIP, CoIP, IF, IHC, IP, IP-MS, RIP, WB|
|Tested Reactivity||aequorea victoria, recombinant protein|
|Immunogen||GFP tag fusion protein Ag2128|
|Host / Isotype||Rabbit / IgG|
|Full Name||GFP tag|
|Synonyms||CFP, eGFP, eYFP, GFP, GFP tag, YFP|
|Calculated molecular weight||26 kDa|
|GenBank accession number||U73901|
|Gene ID (NCBI)|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
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