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GDNF Recombinant antibody

GDNF Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230358A10

Cat No : 83087-1-RR

Print datasheet

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

验证数据展示

  • FC experiment of SH-SY5Y using 83087-1-RR

    FC experiment of SH-SY5Y using 83087-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inSH-SY5Y cells, U-251 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83087-1-RR targets GDNF in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA
Tested Reactivity Human
Immunogen GDNF fusion protein Ag24068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glial cell derived neurotrophic factor
Synonyms ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 32-40 kDa
GenBank Accession NumberBC069369
Gene Symbol GDNF
Gene ID (NCBI) 2668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP39905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

实验方案

Product Specific Protocols
FC protocol for GDNF antibody 83087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols