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验证数据展示

  • IHC staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    IHC staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    IHC staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83087-1-RR (GDNF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83087-1-RR (same clone as 83087-1-PBS)

    FC experiment of U-251 using 83087-1-RR (same clone as 83087-1-PBS)

    (blue)1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83087-1-RR (same clone as 83087-1-PBS)

    FC experiment of SH-SY5Y using 83087-1-RR (same clone as 83087-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    IF Staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    IF Staining of mouse brain using 83087-1-RR (same clone as 83087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 83087-1-RR (same clone as 83087-1-PBS)

    IF Staining of SH-SY5Y using 83087-1-RR (same clone as 83087-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GDNF antibody (83087-1-RR, Clone: 230358A10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83087-1-PBS in a different storage buffer formulation.

GDNF Recombinant monoclonal antibody, PBS Only

GDNF Uni-rAb® Recombinant Antibody for IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 83087-1-PBS

产品说明书

CloneNo. 230358A10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

230358A10, Astrocyte-derived trophic factor, ATF, ATF1, ATF2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83087-1-PBS targets GDNF in IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24068

Product name: Recombinant human GDNF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 78-211 aa of BC069369

Sequence: SPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glial cell derived neurotrophic factor
别名 230358A10, Astrocyte-derived trophic factor, ATF, ATF1, ATF2
计算分子量 211 aa, 24 kDa
观测分子量 32-40 kDa
GenBank蛋白编号BC069369
基因名称 GDNF
Gene ID (NCBI) 2668
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP39905
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

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