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验证数据展示

  • WB analysis using 12601-1-AP (same clone as 12601-1-PBS)

    WB analysis using 12601-1-AP (same clone as 12601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

    IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

    IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

    IP experiment of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

    IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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GCLC Polyclonal antibody, PBS Only

GCLC Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12601-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12601-1-PBS targets GCLC in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3252

Product name: Recombinant human GCLC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-252 aa of BC022487

Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate-cysteine ligase, catalytic subunit
别名 EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC
计算分子量 637 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC022487
基因名称 GCLC
Gene ID (NCBI) 2729
RRIDAB_2278734
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48506
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

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