验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, HEK-293 cells, L02 cells, mouse kidney tissue, Jurkat cells, Raji cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12601-1-AP targets GCLC in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 |
CatNo: Ag3252 Product name: Recombinant human GCLC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC022487 Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamate-cysteine ligase, catalytic subunit |
| 别名 | EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC |
| 计算分子量 | 637 aa, 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC022487 |
| 基因名称 | GCLC |
| Gene ID (NCBI) | 2729 |
| RRID | AB_2278734 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48506 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCLC antibody 12601-1-AP | Download protocol |
| IP protocol for GCLC antibody 12601-1-AP | Download protocol |
| WB protocol for GCLC antibody 12601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.
| ||
Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. | ||
Hepatology Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury. |
