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验证数据展示

  • WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

    WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32217-1-AP (GCC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

  • WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

    WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32217-1-AP (GCC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 32217-1-AP (same clone as 32217-1-PBS)

    IHC staining of human stomach cancer using 32217-1-AP (same clone as 32217-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 32217-1-AP (GCC2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

GCC2 Polyclonal antibody, PBS Only

GCC2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32217-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC185, GRIP And Coiled-Coil Domain Containing 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32217-1-PBS targets GCC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17535

Product name: Recombinant human GCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-343 aa of BC146789

Sequence: MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRIP and coiled-coil domain containing 2
别名 185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC185, GRIP And Coiled-Coil Domain Containing 2
计算分子量 1583 aa, 185 kDa
观测分子量200 kDa
GenBank蛋白编号BC146789
基因名称 GCC2
Gene ID (NCBI) 9648
RRIDAB_3742546
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8IWJ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The GCC2 protein, fully named GRIP and Coiled-Coil Domain-Containing Protein 2, is a key adaptor protein in the intracellular Golgi-endosomal membrane transport system. It specifically anchors to the Golgi membrane via its GRIP domain and is primarily responsible for mediating retrograde vesicular transport from endosomes to the Golgi apparatus, participating in the recycling and sorting of various cargo proteins, such as the mannose-6-phosphate receptor. Recent studies have revealed that GCC2 regulates the subcellular localization of the GATOR complex, thereby acting as an important upstream regulator of the mTORC1 signaling pathway, which influences cell growth, metabolism, and autophagy. Dysfunction of GCC2 is closely associated with neurodevelopmental disorders and tumorigenesis, underscoring its significance in cell biology and disease research.

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