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验证数据展示

  • WB analysis using 25409-1-AP (same clone as 25409-1-PBS)

    WB analysis using 25409-1-AP (same clone as 25409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25409-1-AP (GAS2L3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 25409-1-AP (same clone as 25409-1-PBS)

    IP experiment of HEK-293 using 25409-1-AP (same clone as 25409-1-PBS)

    IP result of anti-GAS2L3 (IP:25409-1-AP, 4ug; Detection:25409-1-AP 1:1000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)

    IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25409-1-AP (GAS2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)

    IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25409-1-AP (GAS2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.

GAS2L3 Polyclonal antibody, PBS Only

GAS2L3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25409-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

GAS2 like protein 3, growth arrest 2 like 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25409-1-PBS targets GAS2L3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22097

Product name: Recombinant human GAS2L3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 347-694 aa of BC043366

Sequence: GRPPGALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPALPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLAAHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNLNQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPKSAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth arrest-specific 2 like 3
别名 GAS2 like protein 3, growth arrest 2 like 3
计算分子量 694 aa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC043366
基因名称 GAS2L3
Gene ID (NCBI) 283431
RRIDAB_3669473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XJ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAS2L3 also known as G2L3, belongs to the GAS2 family of four related proteins. It contains an N-terminal calponin homology (CH) domain and a C-terminal GAS2-related (GAR) domain, which are putative actin and microtubule binding domains. GAS2L3 is specifically expressed in mitosis and it localizes to the midbody and the constriction zone during cytokinesis (PMID: 28937871).

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