验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25409-1-AP targets GAS2L3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22097 Product name: Recombinant human GAS2L3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 347-694 aa of BC043366 Sequence: GRPPGALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPALPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLAAHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNLNQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPKSAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth arrest-specific 2 like 3 |
| 别名 | GAS2 like protein 3, growth arrest 2 like 3 |
| 计算分子量 | 694 aa, 75 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC043366 |
| 基因名称 | GAS2L3 |
| Gene ID (NCBI) | 283431 |
| RRID | AB_3669473 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86XJ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAS2L3 also known as G2L3, belongs to the GAS2 family of four related proteins. It contains an N-terminal calponin homology (CH) domain and a C-terminal GAS2-related (GAR) domain, which are putative actin and microtubule binding domains. GAS2L3 is specifically expressed in mitosis and it localizes to the midbody and the constriction zone during cytokinesis (PMID: 28937871).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAS2L3 antibody 25409-1-AP | Download protocol |
| IP protocol for GAS2L3 antibody 25409-1-AP | Download protocol |
| WB protocol for GAS2L3 antibody 25409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
