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HRP-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (7)

Applications

WB and More (3)

Conjugate

HRP

CloneNo.

1E6D9

Cat no : HRP-60004

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-60004 targets GAPDH in WB, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Applications WB Application Description
Cited ApplicationsWB, IP, CoIP, RIP
Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, chicken, hamster, opossum, honey bees
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2737588
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04406
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for HRP GAPDH antibody HRP-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Authors - Linbin Yang
humanWB

Mol Cancer

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Authors - Rashmi Bharti
humanWB

Nat Biotechnol

Direct targeting of amplified gene loci for proapoptotic anticancer therapy.

Authors - Meetu Kaushik Tiwari
humanWB

Cell

Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.

Authors - Simone Rauch
humanWB

Cell

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Authors - Shicheng Su
humanWB

Cell

CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness.

Authors - Shicheng Su