CoraLite® Plus 488-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, yeast, plant

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E6D9

Cat No : CL488-60004

Print datasheet

Synonyms

glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60004-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 7 publications below

产品信息

CL488-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.

Tested Applications WB, FC (Intra) Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, zebrafish, yeast, plant
Cited Reactivityhuman, mouse, rat
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2919223
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04406
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 GAPDH antibody CL488-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation.

Authors - Guojun Gao
humanWB

Cell Commun Signal

Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.

Authors - Cefan Zhou
humanWB

Sci Rep

Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD.

Authors - Shuang Bai
humanWB

Exp Cell Res

CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.

Authors - Decai Chi
ratWB

Virus Res

Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin.

Authors - Julie A Hicks
mouseWB

Reprod Fertil Dev

Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.

Authors - Jian-Mei Zhang