CoraLite® Plus 488-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b


human, mouse, rat, zebrafish, yeast, plant


WB, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye



Cat No : CL488-60004

Print datasheet


glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:, CDABP0047


Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60004-1-Ig


Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


WBSee 7 publications below


CL488-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.

Tested Applications WB, FC (Intra) Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, zebrafish, yeast, plant
Cited Reactivityhuman, mouse, rat
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:, CDABP0047
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.


Product Specific Protocols
WB protocol for CL Plus 488 GAPDH antibody CL488-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols



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