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验证数据展示

  • IP experiment of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    IP experiment of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    IP result of anti-G6PC2 (IP:18074-1-AP, 4ug; Detection:18074-1-AP 1:500) with mouse pancreas tissue lysate 2480 ug. This data was developed using the same antibody clone with 18074-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    IHC staining of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18074-1-AP (G6PC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18074-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    IF Staining of mouse pancreas using 18074-1-AP (same clone as 18074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using G6PC2 antibody (18074-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18074-1-PBS in a different storage buffer formulation.

G6PC2 Polyclonal antibody, PBS Only

G6PC2 Polyclonal Antibody for IHC, IF-P, IP, Indirect ELISA
Cat No. 18074-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, IP, Indirect ELISA

别名

G6Pase 2, IGRP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18074-1-PBS targets G6PC2 in IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12764

Product name: Recombinant human G6PC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC104778

Sequence: MDFLHRNGVLIIQHLQKDYRAYYTFLNFMSNVGDPRNIFFIYFPLCFQFNQTVGTKMIWVAVIGDWLNLIFKWILFGHRPYWWVQETQIYPNHSSPCLEQFPTTCETGPGSPSGHAMGASCVWYVMVTAALSHTVCGMDKFSITLHRLTWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucose-6-phosphatase, catalytic, 2
别名 G6Pase 2, IGRP
计算分子量 355 aa, 41 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC104778
基因名称 G6PC2
Gene ID (NCBI) 57818
RRIDAB_3741945
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQR9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

G6PC2, also known as IGRP, is a member of the glucose-6-phosphatase catalytic subunit (G6PC) gene family. G6PC2 catalyzes the hydrolysis of glucose-6-phosphate to glucose and inorganic phosphate within the lumen of the endoplasmic reticulum. G6PC2 is mainly expressed in pancreatic islet β-cells. G6PC2 acts to oppose the action of the beta cell glucose sensor, glucokinase, by creating a futile substrate cycle. (PMID: 37855366, PMID: 32569842, PMID: 37552875)

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