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CoraLite®594-conjugated Frizzled 9 Monoclonal antibody

Frizzled 9 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G9G4

Cat No : CL594-67023

Print datasheet

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

验证数据展示

  • FC experiment of HeLa using CL594-67023

    FC experiment of HeLa using CL594-67023

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Frizzled 9 (CL594-67023, Clone:1G9G4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67023-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67023 targets Frizzled 9 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen Frizzled 9 fusion protein Ag4971 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name frizzled homolog 9 (Drosophila)
Synonyms CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9
Calculated Molecular Weight 591 aa, 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC026333
Gene Symbol FZD9
Gene ID (NCBI) 8326
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL594 Frizzled 9 antibody CL594-67023Download protocol
Standard Protocols
Click here to view our Standard Protocols