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验证数据展示

  • IF Staining of HEK-293 using CL594-67023

    IF Staining of HEK-293 using CL594-67023

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 Frizzled 9 antibody (CL594-67023, Clone: 1G9G4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67023

    FC experiment of HeLa using CL594-67023

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Frizzled 9 (CL594-67023, Clone:1G9G4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Frizzled 9 Monoclonal antibody

Frizzled 9 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67023

产品说明书

CloneNo. 1G9G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

FZD9, FZD3, Fz-9, Fz 9, Frizzled-9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67023-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67023 targets Frizzled 9 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Frizzled 9 fusion protein Ag4971 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 frizzled homolog 9 (Drosophila)
别名 FZD9, FZD3, Fz-9, Fz 9, Frizzled-9
计算分子量 591 aa, 64 kDa
观测分子量 64-70 kDa
GenBank蛋白编号BC026333
基因名称 Frizzled 9
Gene ID (NCBI) 8326
RRIDAB_2934756
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00144
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Frizzled 9 antibody CL594-67023Download protocol
Standard Protocols
Click here to view our Standard Protocols