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验证数据展示

  • WB analysis of A549 using 67023-1-Ig

    WB analysis of A549 using 67023-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67023-1-Ig

    IF Staining of HEK-293 using 67023-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Frizzled 9 antibody (67023-1-Ig, Clone: 1G9G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human gliomas using 67023-1-Ig

    IHC staining of human gliomas using 67023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 67023-1-Ig

    IHC staining of human gliomas using 67023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Frizzled 9 Monoclonal antibody

Frizzled 9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67023-1-Ig

产品说明书

CloneNo. 1G9G4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HUVEC cells, NCI-H1299 cells, fetal human brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67023-1-Ig targets Frizzled 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 Frizzled 9 fusion protein Ag4971 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 frizzled homolog 9 (Drosophila)
别名 CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9
计算分子量 591 aa, 64 kDa
观测分子量 64-70 kDa
GenBank蛋白编号BC026333
基因名称 Frizzled 9
Gene ID (NCBI) 8326
RRIDAB_2882338
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
IHC protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
IF protocol for Frizzled 9 antibody 67023-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Authors - Jasjot Singh
View Article