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验证数据展示

  • WB analysis using 24272-1-AP (same clone as 24272-1-PBS)

    WB analysis using 24272-1-AP (same clone as 24272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24272-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 24272-1-AP (same clone as 24272-1-PBS)

    IHC staining of mouse colon using 24272-1-AP (same clone as 24272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24272-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 24272-1-AP (same clone as 24272-1-PBS)

    IHC staining of mouse colon using 24272-1-AP (same clone as 24272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24272-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Frizzled 2 Polyclonal antibody, PBS Only

Frizzled 2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24272-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

FZD2, Frizzled-2, Fz 2, Fz-2, FzE2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24272-1-PBS targets Frizzled 2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18003

Product name: Recombinant human FZD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 138-217 aa of BC113402

Sequence: CEHFPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 frizzled homolog 2 (Drosophila)
别名 FZD2, Frizzled-2, Fz 2, Fz-2, FzE2
计算分子量 565 aa, 64 kDa
观测分子量 64-70 kDa
GenBank蛋白编号BC113402
基因名称 Frizzled 2
Gene ID (NCBI) 2535
RRIDAB_2879463
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14332
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Frizzled 2(FZD2) also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. FZD2 is an important receptor in the Wnt pathway, which is highly expressed in malignant tumors and helps regulate multiple tumor behaviors. Its expression level is related to prognosis. Moreover, high level of FZD2 had significant correlation with poor prognosis in Breast cancer (BC) patients(PMID: 33832493).

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