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验证数据展示

  • WB analysis using 86959-3-RR

    WB analysis using 86959-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86959-3-RR (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86959-3-RR

    IF Staining of HeLa using 86959-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Filamin A antibody (86959-3-RR, Clone: 252084C12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86959-3-RR

    Affinity and Kinetic Characterization of 86959-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86959-3-RR against Human Filamin A were performed. The affinity constant is 6.35 nM.

Filamin A Recombinant monoclonal antibody

Filamin A Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86959-3-RR

产品说明书

CloneNo. 252084C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

FLNA, ABP-280, Actin-binding protein 280, Alpha filamin, Alpha-filamin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FLNA Ready-To-Use IHC Kit. FLNA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86959-3-RR targets Filamin A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1581

Product name: Recombinant human FLNA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2348-2647 aa of BC014654

Sequence: NQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 filamin A, alpha (actin binding protein 280)
别名 FLNA, ABP-280, Actin-binding protein 280, Alpha filamin, Alpha-filamin
计算分子量 2647 aa, 280 kDa
观测分子量 280 kDa
GenBank蛋白编号BC014654
基因名称 FLNA
Gene ID (NCBI) 2316
RRIDAB_3745235
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21333
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)

实验方案

Product Specific Protocols
IF protocol for Filamin A antibody 86959-3-RRDownload protocol
WB protocol for Filamin A antibody 86959-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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