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验证数据展示

  • FC experiment of C2C12 using CL594-67150

    FC experiment of C2C12 using CL594-67150

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human FXYD1 (CL594-67150, Clone:2C10G9) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67150

产品说明书

CloneNo. 2C10G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Pig, mouse

应用

FC (Intra)

别名

FXYD1, Phospholemman, PLM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inC2C12 cells
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy FXYD1 Ready-To-Use IHC Kit. FXYD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67150-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67150 targets FXYD1 in FC (Intra) applications and shows reactivity with Human, Pig, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Pig, mouse
免疫原

CatNo: Ag25307

Product name: Recombinant human FXYD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-92 aa of BC032800

Sequence: MASLGHILVFCVGLLTMAKAESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 1
别名 FXYD1, Phospholemman, PLM
计算分子量 92 aa, 10 kDa
观测分子量 15 kDa
GenBank蛋白编号BC032800
基因名称 FXYD1
Gene ID (NCBI) 5348
RRIDAB_3084754
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00168
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

实验方案

Product Specific Protocols
FC protocol for CL594 FXYD1 antibody CL594-67150Download protocol
Standard Protocols
Click here to view our Standard Protocols