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验证数据展示

  • WB analysis of human heart using 67150-1-Ig

    WB analysis of human heart using 67150-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 67150-1-Ig

    IHC staining of human heart using 67150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FXYD1 antibody included
  • IHC staining of human heart using 67150-1-Ig

    IHC staining of human heart using 67150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FXYD1 antibody included
  • IF Staining of mouse heart using 67150-1-Ig

    IF Staining of mouse heart using 67150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse heart using 67150-1-Ig

    IF Staining of mouse heart using 67150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67150-1-Ig

产品说明书

CloneNo. 2C10G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Pig, mouse

应用

WB, IHC, IF-P, ELISA

别名

FXYD1, Phospholemman, PLM

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inhuman heart tissue, human skeletal muscle tissue, pig heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy FXYD1 Ready-To-Use IHC Kit. FXYD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67150-1-Ig targets FXYD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Pig, mouse
免疫原

CatNo: Ag25307

Product name: Recombinant human FXYD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-92 aa of BC032800

Sequence: MASLGHILVFCVGLLTMAKAESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 1
别名 FXYD1, Phospholemman, PLM
计算分子量 92 aa, 10 kDa
观测分子量 15 kDa
GenBank蛋白编号BC032800
基因名称 FXYD1
Gene ID (NCBI) 5348
RRIDAB_2882448
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00168
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

实验方案

Product Specific Protocols
IF protocol for FXYD1 antibody 67150-1-IgDownload protocol
IHC protocol for FXYD1 antibody 67150-1-IgDownload protocol
WB protocol for FXYD1 antibody 67150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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