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  • Featured Product
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CoraLite® Plus 488-conjugated FUT9 Monoclonal antibody

FUT9 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5D4G6

Cat No : CL488-60230

Print datasheet

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

验证数据展示

  • IF Staining of MCF-7 using CL488-60230

    IF Staining of MCF-7 using CL488-60230

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 FUT9 antibody (CL488-60230, Clone: 5D4G6 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 60230-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60230 targets FUT9 in IF applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, pig, mouse, rat
Immunogen FUT9 fusion protein Ag8298 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
Synonyms Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9
Calculated Molecular Weight 359 aa, 42 kDa
Observed Molecular Weight 44-46 kDa
GenBank Accession NumberBC036101
Gene Symbol FUT9
Gene ID (NCBI) 10690
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y231
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FUT9 antibody CL488-60230Download protocol
Standard Protocols
Click here to view our Standard Protocols