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- KD/KO Validated
FUT9 Monoclonal antibody
FUT9 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig, mouse, rat
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5D4G6
验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Positive IHC detected in | human stomach tissue, human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
The immunogen of 60230-1-Ig is FUT9 Fusion Protein expressed in E. coli.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | FUT9 fusion protein Ag8298 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fucosyltransferase 9 (alpha (1,3) fucosyltransferase) |
| Synonyms | Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9 |
| Calculated Molecular Weight | 359 aa, 42 kDa |
| Observed Molecular Weight | 44-46 kDa |
| GenBank Accession Number | BC036101 |
| Gene Symbol | FUT9 |
| Gene ID (NCBI) | 10690 |
| RRID | AB_11183046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IHC protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IF protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
| ||
Acta Biochim Biophys Sin (Shanghai) ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9
|
