CoraLite®594-conjugated FUCA1 Monoclonal antibody

FUCA1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5C5

Cat No : CL594-67251

Print datasheet

Synonyms

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase



经过测试的应用

Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy FUCA1 Ready-To-Use IHC Kit. FUCA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67251-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67251 targets FUCA1 in IF applications and shows reactivity with Human, Mouse samples.

Tested Applications IF-P
Tested Reactivity Human, Mouse
Immunogen FUCA1 fusion protein Ag9638 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fucosidase, alpha-L- 1, tissue
Synonyms Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase
Calculated Molecular Weight 461 aa, 53 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC017338
Gene Symbol FUCA1
Gene ID (NCBI) 2517
RRIDAB_2920103
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04066
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

实验方案

Product Specific Protocols
IF protocol for CL594 FUCA1 antibody CL594-67251Download protocol
Standard Protocols
Click here to view our Standard Protocols