CoraLite®594-conjugated FUCA1 Monoclonal antibody
FUCA1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G5C5
验证数据展示
经过测试的应用
Positive IF detected in | human liver cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy FUCA1 Ready-To-Use IHC Kit. FUCA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67251-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67251 targets FUCA1 in IF applications and shows reactivity with Human, Mouse samples.
Tested Applications | IF-P |
Tested Reactivity | Human, Mouse |
Immunogen | FUCA1 fusion protein Ag9638 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | fucosidase, alpha-L- 1, tissue |
Synonyms | Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase |
Calculated Molecular Weight | 461 aa, 53 kDa |
Observed Molecular Weight | 50-56 kDa |
GenBank Accession Number | BC017338 |
Gene Symbol | FUCA1 |
Gene ID (NCBI) | 2517 |
RRID | AB_2920103 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04066 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 FUCA1 antibody CL594-67251 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |