验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy FTSJ3 Ready-To-Use IHC Kit. FTSJ3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84311-6-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84311-6 targets FTSJ3 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9835 Product name: Recombinant human FTSJ3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 491-847 aa of BC036710 Sequence: KRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | FtsJ homolog 3 (E. coli) |
| 别名 | 241507F2, EC:2.1.1.-, EPCS3, FTSJ 3, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 |
| 计算分子量 | 847 aa, 97 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | BC036710 |
| 基因名称 | FTSJ3 |
| Gene ID (NCBI) | 117246 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8IY81 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FTSJ3 antibody CL488-84311-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
