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验证数据展示

  • WB analysis using 84311-6-RR

    WB analysis using 84311-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84311-6-RR (FTSJ3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84311-6-RR

    IF Staining of MCF-7 using 84311-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FTSJ3 antibody (84311-6-RR, Clone: 241507F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84311-6-RR

    Affinity and Kinetic Characterization of 84311-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84311-6-RR against Human FTSJ3 were performed. The affinity constant is 0.68 nM.

FTSJ3 Recombinant monoclonal antibody

FTSJ3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84311-6-RR

产品说明书

CloneNo. 241507F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SB92, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, FTSJ 3, EPCS3, EC:2.1.1.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, SH-SY5Y cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FTSJ3 Ready-To-Use IHC Kit. FTSJ3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84311-6-RR targets FTSJ3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9835

Product name: Recombinant human FTSJ3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 491-847 aa of BC036710

Sequence: KRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FtsJ homolog 3 (E. coli)
别名 SB92, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, FTSJ 3, EPCS3, EC:2.1.1.-
计算分子量 847 aa, 97 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC036710
基因名称 FTSJ3
Gene ID (NCBI) 117246
RRIDAB_3671851
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IY81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

实验方案

Product Specific Protocols
IF protocol for FTSJ3 antibody 84311-6-RRDownload protocol
WB protocol for FTSJ3 antibody 84311-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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