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FTO Recombinant antibody

FTO Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat No : 81471-1-RR

Print datasheet

Synonyms

FTO, KIAA1752

验证数据展示

  • WB analysis using 81471-1-RR

    WB analysis using 81471-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81471-1-RR

    IHC staining of human liver cancer using 81471-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FTO antibody included
  • IF Staining of HepG2 using 81471-1-RR

    IF Staining of HepG2 using 81471-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81471-1-RR targets FTO in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen FTO fusion protein Ag26095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fat mass and obesity associated
Synonyms FTO, KIAA1752
Calculated Molecular Weight 58 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9C0B1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

实验方案

Product Specific Protocols
WB protocol for FTO antibody 81471-1-RRDownload protocol
IHC protocol for FTO antibody 81471-1-RRDownload protocol
IF protocol for FTO antibody 81471-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols