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验证数据展示

  • WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • WB analysis of MDA-MB-231 using 81471-1-RR (same clone as 81471-1-PBS)

    WB analysis of MDA-MB-231 using 81471-1-RR (same clone as 81471-1-PBS)

    WB result of FTO antibody (81471-1-RR; 1:600; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 81471-1-RR (same clone as 81471-1-PBS)

    FC experiment of NIH/3T3 using 81471-1-RR (same clone as 81471-1-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug FTO Recombinant antibody (81471-1-RR, Clone:4L4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FTO Recombinant monoclonal antibody, PBS Only

FTO Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81471-1-PBS

产品说明书

CloneNo. 4L4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

4L4, KIAA1752

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

产品信息

81471-1-PBS targets FTO in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26095

Product name: Recombinant human FTO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-505 aa of NM_001080432

Sequence: MAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fat mass and obesity associated
别名 4L4, KIAA1752
计算分子量 58 kDa
观测分子量58 kDa
GenBank蛋白编号NM_001080432
基因名称 FTO
Gene ID (NCBI) 79068
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0B1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in th1e FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

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