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验证数据展示

  • WB analysis using 60635-1-Ig (same clone as 60635-1-PBS)

    WB analysis using 60635-1-Ig (same clone as 60635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60635-1-Ig (FPGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60635-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60635-1-Ig (same clone as 60635-1-PBS)

    FC experiment of HeLa using 60635-1-Ig (same clone as 60635-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FPGS Monoclonal antibody (60635-1-Ig, Clone:2D10H11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 60635-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50907-1

    Cytometric bead array standard curve of MP50907-1

    Cytometric bead array standard curve of MP50907-1, FPGS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60635-1-PBS. Detection antibody: 60635-2-PBS. Standard:Ag36736. Range: 0.781-100 ng/mL.

FPGS Monoclonal antibody, PBS Only (Capture)

FPGS Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60635-1-PBS

产品说明书

CloneNo. 2D10H11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

EC:6.3.2.17, Folylpoly-gamma-glutamate synthetase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60635-1-PBS targets FPGS as part of a matched antibody pair:

MP50907-1: 60635-1-PBS capture and 60635-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36736

Product name: Recombinant human FPGS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 236-587 aa of BC064393

Sequence: VEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYLCPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVLRRGPLTWYLDGAHTASSAQACVRWFRQALQGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSVPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 folylpolyglutamate synthase
别名 EC:6.3.2.17, Folylpoly-gamma-glutamate synthetase
计算分子量 65 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC064393
基因名称 FPGS
Gene ID (NCBI) 2356
RRIDAB_3743226
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ05932
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

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