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验证数据展示

  • WB analysis using 60635-1-Ig

    WB analysis using 60635-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60635-1-Ig (FPGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 60635-1-Ig

    FC experiment of HeLa using 60635-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FPGS Monoclonal antibody (60635-1-Ig, Clone:2D10H11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

FPGS Monoclonal antibody

FPGS Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 60635-1-Ig

产品说明书

CloneNo. 2D10H11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

EC:6.3.2.17, Folylpoly-gamma-glutamate synthetase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60635-1-Ig targets FPGS in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36736

Product name: Recombinant human FPGS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 236-587 aa of BC064393

Sequence: VEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYLCPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVLRRGPLTWYLDGAHTASSAQACVRWFRQALQGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSVPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 folylpolyglutamate synthase
别名 EC:6.3.2.17, Folylpoly-gamma-glutamate synthetase
计算分子量 65 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC064393
基因名称 FPGS
Gene ID (NCBI) 2356
RRIDAB_3743226
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ05932
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

实验方案

Product Specific Protocols
FC protocol for FPGS antibody 60635-1-IgDownload protocol
WB protocol for FPGS antibody 60635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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