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验证数据展示

  • WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

    WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

    WB result of FOXM1 antibody (13147-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXM1 transfected HeLa cells. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

  • WB analysis using 13147-1-AP (same clone as 13147-1-PBS)

    WB analysis using 13147-1-AP (same clone as 13147-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

    WB analysis of HeLa using 13147-1-AP (same clone as 13147-1-PBS)

    Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 13147-1-AP (same clone as 13147-1-PBS)

    IP experiment of L02 using 13147-1-AP (same clone as 13147-1-PBS)

    IP result of anti-FOXM1 (IP:13147-1-AP, 4ug; Detection:13147-1-AP 1:1000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 13147-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FOXM1 Polyclonal antibody, PBS Only

FOXM1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13147-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

M-phase phosphoprotein 2, HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13147-1-PBS targets FOXM1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3729

Product name: Recombinant human FOXM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 447-748 aa of BC006192

Sequence: EEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTPQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box M 1
别名 M-phase phosphoprotein 2, HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16
计算分子量 83 kDa
观测分子量 84-110 kDa
GenBank蛋白编号BC006192
基因名称 FOXM1
Gene ID (NCBI) 2305
RRIDAB_2106213
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08050
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

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