验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, HEK-293 cells, HeLa cells, human testis tissue, mouse colon tissue, mouse spleen tissue, mouse testis tissue, mouse thymus tissue, MCF-7 cells, L02 cells, A431 cells |
| Positive IP detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13147-1-AP targets FOXM1 in WB, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP, chIP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | FOXM1 fusion protein Ag3729 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box M 1 |
| 别名 | M-phase phosphoprotein 2, HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16 |
| 计算分子量 | 83 kDa |
| 观测分子量 | 84-110 kDa |
| GenBank蛋白编号 | BC006192 |
| 基因名称 | FOXM1 |
| Gene ID (NCBI) | 2305 |
| RRID | AB_2106213 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08050 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| IP protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.
| ||
Nat Commun K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis | ||
Acta Pharm Sin B Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation. | ||
Nat Commun Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation. | ||
Nat Commun FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence. | ||
Nat Commun circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.
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