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验证数据展示

  • WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

    WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66548-1-Ig (FMR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

    IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

    IP result of anti-FMR1 (IP:66548-1-Ig, 5ug; Detection:66548-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

    IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FMR1 antibody included
  • FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

    FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FMR1 Monoclonal antibody, PBS Only

FMR1 Monoclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 66548-1-PBS

产品说明书

CloneNo. 2B5B5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

2B5B5, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.

产品信息

66548-1-PBS targets FMR1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4697

Product name: Recombinant human FMR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC038998

Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fragile X mental retardation 1
别名 2B5B5, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1
计算分子量 632 aa, 71 kDa
观测分子量 69-72 kDa
GenBank蛋白编号BC038998
基因名称 FMR1
Gene ID (NCBI) 2332
RRIDAB_2881910
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ06787
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

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