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验证数据展示

  • WB analysis using 85931-1-RR

    WB analysis using 85931-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 85931-1-RR

    IHC staining of mouse lung using 85931-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 85931-1-RR

    IHC staining of mouse lung using 85931-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEL using 85931-1-RR

    IF Staining of HEL using 85931-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

FLI1 Recombinant antibody

FLI1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85931-1-RR

产品说明书

CloneNo. 250294F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, U-937 cells, A20 cells, RAW 264.7 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEL cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85931-1-RR targets FLI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FLI1 fusion protein Ag36169 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Friend leukemia virus integration 1
别名 FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1
计算分子量 452 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC010115
基因名称 FLI1
Gene ID (NCBI) 2313
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

实验方案

Product Specific Protocols
WB protocol for FLI1 antibody 85931-1-RRDownload protocol
IHC protocol for FLI1 antibody 85931-1-RRDownload protocol
IF protocol for FLI1 antibody 85931-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols