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验证数据展示

  • WB analysis using 85931-1-RR (same clone as 85931-1-PBS)

    WB analysis using 85931-1-RR (same clone as 85931-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

    IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

    IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

  • IF Staining of HEL using 85931-1-RR (same clone as 85931-1-PBS)

    IF Staining of HEL using 85931-1-RR (same clone as 85931-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

  • Sample test of MP02198-1

    Sample test of MP02198-1

    The mean FLI1 concentration was determined to be 303.2 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP02198-1

    Cytometric bead array standard curve of MP02198-1

    Cytometric bead array standard curve of MP02198-1, FLI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85931-3-PBS. Detection antibody: 85931-1-PBS. Standard: Ag36169. Range: 0.781-100 ng/mL.

FLI1 Recombinant antibody, PBS Only (Detector)

FLI1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 85931-1-PBS

产品说明书

CloneNo. 250294F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85931-1-PBS targets FLI1 as part of a matched antibody pair:

MP02198-1: 85931-3-PBS capture and 85931-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 FLI1 fusion protein Ag36169 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Friend leukemia virus integration 1
别名 FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1
计算分子量 452 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC010115
基因名称 FLI1
Gene ID (NCBI) 2313
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01543
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.