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FBP1 Monoclonal antibody

FBP1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G4E4

Cat No : 68446-1-Ig

Print datasheet

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

验证数据展示

  • WB analysis using 68446-1-Ig

    WB analysis using 68446-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 68446-1-Ig

    IHC staining of mouse kidney using 68446-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBP1 antibody included
  • IHC staining of mouse kidney using 68446-1-Ig

    IHC staining of mouse kidney using 68446-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBP1 antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, MCF-7 cells, T-47D cells, pig liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FBP1 Ready-To-Use IHC Kit. FBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68446-1-Ig targets FBP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen FBP1 fusion protein Ag3837 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fructose-1,6-bisphosphatase 1
Synonyms FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC012927
Gene Symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09467
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

实验方案

Product Specific Protocols
WB protocol for FBP1 antibody 68446-1-IgDownload protocol
IHC protocol for FBP1 antibody 68446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols